Structural studies of ß-turn-containing peptide catalysts for atroposelective quinazolinone bromination.

We describe herein a crystallographic and NMR study of the secondary structural attributes of a ß-turn-containing tetra-peptide, Boc-Dmaa-D-Pro-Acpc-Leu-NMe2, which was recently reported as a highly effective catalyst in the atroposelective bromination of 3-arylquinazolin-4(3H)-ones. Inquiries pertaining to the functional consequences of residue substitutions led to the discovery of a more selective catalyst, Boc-Dmaa-D-Pro-Acpc-Leu-OMe, the structure of which was also explored. This new lead catalyst was found to exhibit a type I'ß-turn secondary structure both in the solid state and in solution, a structure that was shown to be an accessible conformation of the previously reported catalyst, as well.

[Automation of parenteral nutrition elaboration: adaptation to in force legislation]. / Automatización de la elaboración de nutrición parenteral: adecuación a la legislación actual.

INTRODUCTION: Quality and efficiency criteria of allowances associated to technological procedures are developing in a setting that values quality, and from there the Real Decree RD175/2001 has been issued by which the rules of correct elaboration and quality control of formulations and pharmacy preparations. Parenteral nutrition (PN) is a formulation and, as such, its elaboration and control have to agree with the in force regulations. With this aim, at the Bellvitge University Hospital we have developed a project for automation of elaboration of PN mixtures with the MicroMacro Pump 23 Baxa Compounder. OBJECTIVES: To assess the impact of implementing an automated system of volumetric control in PN elaboration. MATERIAL AND METHODS: The project development may be divided into two differentiated aspects. The first one consisted in the implementation and optimization of the automated system of volumetric control (ASVC). The second aspect comprehends the comparative control performed between the new system and the previous one, and which consisted of a gravimetric system with visual control. For that, real weights were gathered and were compared to predicted weights, and the time of elaboration per bag before and at two times after the implementation of the automated system was registered; the first time just after implementation of the system, and the second time within one year. RESULTS: For the comparison study between both systems used, 141 preparations in total, corresponding to individualized PN, were analyzed: 47 elaborated by means of the gravimetric system (GS), 47 with the ASVC during the implementation period (PN ASVC period 1), 47 PN elaborated with the ASVC within one year of implementation (PN ASVC period 2). The average variation between real weight and predicted weight at the three periods (pre-ASVC, NP ASVC period 1, NP ASVC period 2) was 2.41%, 1.35% and 1.25% respectively (table I). This decrease was statistically significant (p = 0.014). When analyzing the percentage of preparations out of the 3% variation range, we observed a significant reduction (p = 0.00001) by comparing the three periods (Table II). The average elaboration time with the ASVC increased approximately in 4 minutes by bag (3 min 58 sec) during the first studied period, and 3 min 10 sec during the second period, as compared with the study period with the GS. CONCLUSIONS: The new bag-filling system by means of volumetric control represents an improvement in accuracy control and a decrease in the risk for surpassing the acceptable limits. The implementation of a new technological procedure is a difficult task that implies the change of many aspects of the daily practice and that requires a "cultural" change in the PN Unit with the aim of optimizing the process. However, these issues allow for an adaptation to legal requisites in force regarding the regulations of proper elaboration and quality control of formulations as well as an improvement in the integral quality of care.

Implantación y evaluación del análisis de peligros y puntos de control críticos (APPCC) en las industrias tinerfeñas productoras de Gofio / Implementation and evaluation of critical hazards and check points analysis (chcpa) in gofio-producing industries from Tenerife

Objetivo: Implantar de manera satisfactoria el Sistema de Análisis de Peligros y Puntos de Control Críticos. Ámbito: la Isla de Tenerife. Sujetos: 15 industrias. Intervención: se procedió realizando visitas a las fábricas productoras de gofio, con el fin de asesorar a los empresarios y operarios de las mismas, posteriormente se valoró la intervención verificando condiciones higiénico-sanitarias de la industria y la correcta aplicación del Sistema de Autocontrol establecido. Resultados: Después de la intervención de asesoramiento, se observa que determinados parámetros tenidos en cuenta desde el punto de vista higiénico-sanitario se han corregido, como modificar sus instalaciones para adecuarlas a las normativas vigentes o pedir que los proveedores certifiquen las materias primas. En cuanto al proceso de producción del alimento, la intervención fue efectiva para que más de la mitad de las industrias redujeran el tiempo de aquellas fases más susceptibles de contaminación y para que se llevaran a cabo los de registros de control que se establecieron. Conclusiones: Todas las industrias instauraron el sistema de autocontrol mediante cuadros de registros de cada una de las fases de elaboración. El 86% de las industrias han introducido materiales más higiénicos. Un 60% aplicaron una reducción en los plazos de tiempo intermedios en las fases de producción. Un 26% realizaron alguna sustitución de maquinaria obsoleta, modernizando las instalaciones (AU)

Objective: To satisfactorily implement the critical hazards and check points analysis. Setting: Tenerife Island. Subjects: 15 industries. Intervention: visits to gofio-manufacturing industries were done with the aim of giving advice to employers and workers, and thereafter, the intervention was assessed verifying the hygiene and sanitary conditions of the industry and the correct application of the established auto-control system. Results: After the advising intervention, we observed that certain parameters taken into account from the hygiene and sanitary perspective have been corrected, such as modifying the facilities to adapt them to in force regulations, or asking the suppliers to certify raw materials. With regards to food production process, the intervention was effective in such a way that more than have of the industries reduced the time of those phases with higher contamination susceptibility and to carry out the control registries that were established. Conclusions: All industries implemented the autocontrol system by means of registration charts of each one of the elaboration phases. - 86% of the industries have introduced more hygienic materials. - 60% implemented a reduction in intermediate times of production phases. - 26% performed some obsolete machinery replacement modernizing the facilities (AU)